Granuphilin c2a
WebGranuphilin C2A displays a lateral diffusion constant comparable to other C2 domains. However, the diffusion of the granuphilin C2AB tandem on the same membrane appears to be slower; suggesting substantial PS contacts for the C2B domain within the C2AB tandem. This effect represents a weak WebNov 25, 2005 · Deletion analysis showed that the syntaxin-2/3 (or Munc18-1/2)-binding site is a linker domain of Slp4-a (amino acid residues 144-354), a previously uncharacterized region located between the N-terminal Rab27A binding domain and the C2A domain. We also found that the Slp4-a.syntaxin-2 complex is actually present in rat parotid glands …
Granuphilin c2a
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WebOct 12, 2006 · Like granuphilin toward insulin granules, exophilin4 promotes the targeting of glucagon granules to the plasma membrane. Although the interaction of granuphilin with syntaxin-1a is critical for the targeting activity, exophilin4 does this primarily through the affinity of its C2A domain toward the plasma membrane Weband the C2A domains of rabphilins (16). The PIP 2 binding site of the Slp-4 C2A domain was previously reported to center on this cluster (19). Notably, a large region around the …
Webusing site-directed mutagenesis and NMR. Overall, granuphilin demonstrates the capability to bind to background anionic lipids and phosphoinositides inde-pendently, but the presence of both lipids greatly increases the binding affinity of this protein. This suggests that granuphilin may serve as a coincidence de- WebC2 Education is the fastest-growing test prep and tutoring center in the country. Use our Center Locator to help find a center near you and get started!
WebOct 31, 2013 · Granuphilin contains two C2 domains, C2A and C2B, that interact with the plasma membrane lipid phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2]. The goal of …
WebGranuphilin contains two C2 domains, C2A and C2B, that interact with the plasma membrane lipid phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2]. The goal of this …
WebFeb 1, 2015 · The recognition of granuphilin C2A to PI(4,5)P2 within lipid bilayer was analyzed by NMR, using nanodisc system. Abstract. Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) is a key player in regulating the process of excytosis, including insulin secretion. Granuphilin, a tandem C2 domain containing protein, mediates the docking of insulin ... earls restaurant calgary southWebSynaptotagmin-like protein 4 (Slp-4), also known as granuphilin, is a Rab effector responsible for docking secretory vesicles to the plasma membrane before exocytosis. ... earls restaurant downtown denverWebSep 1, 2014 · The granuphilin C2A domain shows significant protein-to-membrane FRET when added to liposomes closely resembling the lipid composition of the plasma … css profile college/program selectionWebHHMI’s Janelia Research Campus in Ashburn, Virginia, cracks open scientific fields by breaking through technical and intellectual barriers. Our integrated teams of lab scientists … css profile create accountWebNov 1, 2013 · Granuphilin contains two C2 domains, C2A and C2B, that interact with the plasma membrane lipid phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2]. The goal of … css profile create parent accountWebRab27a and Rab27b have recently been recognized to play versatile roles in regulating the exocytosis of secretory granules and lysosome-related organelles by using multiple effector proteins. However, the precise roles of these effector proteins in particular cell types largely remain uncharacterized, except for those in pancreatic β cells and in melanocytes. Here, … css profile creatorWebSep 1, 2014 · The granuphilin C2A domain shows significant protein-to-membrane FRET when added to liposomes closely resembling the lipid composition of the plasma membrane cytosolic leaflet (Corbin et al., 2007, Voelker, 2008).Since some C2 domains are known to dock preferentially to PIP x lipids, such physiologically relevant lipid mixtures … css profile currency